Growth and photosynthesis of four invasive aquatic plant species in Europe

Crassula helmsii , Hydrocotyle ranunculoides , Ludwigia grandiflora and Myriophyllum aquaticum are four well known invasive aquatic plants in European waters. In this study, plant growth at different nutrient availabilities, regeneration capacity and photosynthesis were investigated. Results show high relative growth rates (RGR) of the species of up to 0.132 ± 0.008 g g⁻¹ dry weight (dw) day⁻¹ ( H. ranunculoides ) and a significant increase in RGR with increasing nutrient availability. All species show a high regeneration capacity and the ability to form new shoots from single nodes, even though it differs between the species. Ludwigia grandiflora and M. aquaticum also show regeneration from single leaves. Species differed in maximal amounts, and in temperature and light optima of net assimilation rates: H. ranunculoides leaves reach maximum photosynthetic rates of up to 3500 μmol CO₂ × h⁻¹ g⁻¹ dw, L. grandiflora (leaves) up to 2200 μmol CO₂ × h⁻¹ g⁻¹ dw, M. aquaticum (shoots) 400   μmol CO₂ × h⁻¹ g⁻¹ dw and C. helmsii (shoots) up to 200 μmol CO₂ × h⁻¹ g⁻¹ dw. Hydrocotyle ranunculoides , L. grandiflora and M. aquaticum preferred high light intensity and high temperatures, whilst C. helmsii was negatively affected by intense sunlight. Summarising, it can be assumed that at least H. ranunculoides , L. grandiflora and M. aquaticum can grow well under current and likely future central European climate conditions.     

Callus induction and plant regeneration from lateral shoots of herbaceous bamboo Mniochloa abersend

Callus induction and plant regeneration of Mniochloa abersend via lateral shoots were conducted in this study. Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg/L naphthaleneacetic acid (NAA) was effective for compact callus induction. Remarkably, calli on the MS medium with 0.1 mg/L 2,4-D yielded the highest folds of proliferation (8.01), and showed a high potential capacity to differentiate 1 year after subculture. In addition, the compact calli possessed 100% differentiation rate and generated more shoots that were green and strong in 1.0 mg/L kinetin and 1.0 mg/L NAA. Vigorous roots were generated in the 1/2MS supplemented with 0.5 mg/L indole-3-butyric acid, and the resultant plantlets exhibited 90% survival rate after they were hardened and transplanted. The established regeneration system of M. abersend provides a promising platform for bamboo gene function study.     

Analysis of micro-climate on the programs of urban infrastructure regeneration in J city,Republic of Korea

The purpose of this study is to simulate urban micro-climate change due to the variation of wind flow and temperature assuming situations before and after applying programs using urban infrastructure regeneration technologies (UIRTs), and to then quantitatively estimate effectiveness on the urban regeneration schems. UIRTs focus on improving the recycling efficiencies of reclaimed water and biogas produced and to enforce the linkage among all environmental infrastructures associated with water, waste, and energy in an urban block. To implement the micro-climate analyses, data such as land use, topography, building height and materials, and weather conditions were collected, and the results estimated based on fluid simulations of cold wind and temperature variation pathways. There were three programs used in the urban regeneration project in J city: river restoration, traditional market revitalization, and a primary school eco-school. The height of cold wind from the ground level was significantly elevated, and the maximum wind velocity differential between before and after increased by 0.12 m/s due to the expansion of the waterfront space and grassland through the three programs. Overall, a temperature at the ground level dropped by 1.6 °C. Based on these results, the three programs improved the urban environment at ground level, and might ultimately be capable of diminishing the urban heat island effect and mitigating the energy consumption of urban areas.     

不同继代培养次数对苹果八棱海棠离体培养和遗传转化的影响

以苹果八棱海棠离体新梢和幼叶为外植体,研究不同继代次数对其离体繁殖、再生和遗传转化的影响。结果表明,随着继代培养次数的增加,继代10次和48次的八棱海棠离体增殖倍数、生根能力、不定芽的再生频率、每外植体再生芽数和GUS基因瞬时表达率均显著高于继代5次的;继代10次的增殖倍数和GUS阳性率明显多于继代48次的,其它指标相互间差异不显著;说明继代10次的芽苗较适合用于八棱海棠离体培养的外殖体材料,其幼叶较适于遗传转化研究。     

枣离体叶片高效再生植株的研究

 以‘黄骅冬枣’组培苗叶片为试材, 研究了叶片幼嫩程度、叶片来源、组培苗状态以及植物生长调节剂等对离体叶片诱导不定芽再生的影响, 并获得了完整的再生植株。结果表明, 以未生根组培苗中上部叶片再生效果较好; TDZ诱导叶片再生不定芽的效果显著优于BA; 离体叶片在MS + TDZ 1.0 mg·L ^{- 1} + IBA 0.1 mg·L ^{- 1}培养基中诱导培养28 d后, 转入MS + IBA 0.1 mg·L ^{- 1} + GA₃ 0.05 mg·L ^{- 1}培养基中二次培养, 叶片再生效果最好, 再生率可达92.45%。将叶片再生植株转入MS +BA 1.0 mg·L ^{- 1} + KT0.5 mg·L ^{- 1} + IBA 0.1 mg·L ^{- 1}培养基中继代增殖培养, 增殖系数达3.64。以1 /2MS + IAA 1.0 mg·L ^{- 1}培养基诱导生根, 生根率87.1%。生根苗大田移栽成活率达到57%。     

民勤沙区主要植物群落退化特征及其演替趋势分析

优势种植物密度和优势种盖度以及全部植物密度和盖度在样方间均存在不同程度的差异,表明民勤的植物生长在受干旱荒漠环境影响的同时,局部小环境对植物的分布和生长有显著影响。沙蒿当年幼苗密度最大,但幼苗的成活率很低,群落的投影盖度越小,则当年幼苗密度越大;梭梭不能实现自然更新;沙拐枣的生态位宽度较大,各年龄小苗均存在,自然更新能力强。在民勤沙区人工梭梭林普遍退化,尤其在西沙窝一带,梭梭人工林退化更为严重,有被沙拐枣取代的趋势。由于梭梭群落均为人工林,群落内地表异质性较大,因此,草本植物种类较多。     

Effects of vegetation patterns and environmental factors on woody regeneration in semi-arid oak-dominated forests of western Iran

This study assesses the effects of vegetation patterns and environmental factors on the abundance of natural tree and shrub regeneration in semi-arid forests of the Zagros Mountains, western Iran. We sampled 120 releves at different topographic positions in a protected area of the studied region. Floristic composition, slope, elevation and soil properties were recorded at each releve, and woody seedling density was measured. We have first discerned five floristic groups using two-way indicator species analysis(TWINSPAN), detrended correspondence analysis(DCA), and canonical correspondence analysis(CCA) and then explored the relationships among the floristic group compositions, environmental factors and seedling densities. The indicator species of the five groups were Quercus brantii, Acer monspessulanum, Cerasus microcarpa, Rhamnus arvensis and Astragalus licyoides. Our results indicated that these groups were significantly affected by elevation and soil properties and the soil properties refer to: EC(electrical conductivity), N(nitrogen), K(potassium), OM(organic matter), and bulk density. Woody regeneration was composed of Q. brantii, A. monspessulanum, C. microcarpa, Amygdalus scoparia and Crataegus pontica seedlings. The highest density of seedlings was found for Q. brantii(97.14(±48.00) plants/hm~2) and the lowest for A. scoparia(2.28(±1.50) plants/hm~2). Quercus brantii was the dominant species and the seedling density was positively correlated with soil p H and P(phosphorus) values. Amygdalus scoparia regeneration was negatively correlated with elevation, and the seedling density peaked in C. microcarpa group. There was no significant variation in distribution of C. pontica seedlings among the groups, but the seedling density of this species was positively correlated with slope and K. Cerasus microcarpa seedlings were more abundant in the Q. brantii group than in other groups. This study showed that the regeneration of tree and shrub species was unequally distributed in different floristic groups for some species(A. scoparia and C. microcarpa) but not for other(Q. brantii and C. pontica) and was generally correlated with some environmental factors, particularly elevation, slope and soil nutrients(P and K). These results are a first step to implement future management and restoration strategies for promoting forest regeneration.     

不同苜蓿品种对叶片愈伤组织诱导及植株再生的影响

以7个苜蓿品种叶片为外植体,采用3种诱导培养基、5种分化培养基,共15个组合,研究了不同品种间愈伤组织诱导及植株再生的差异。结果表明,不同苜蓿品种间植株再生率差异显著,超级13R在7个苜蓿品种中表现最佳。     

干旱沙区农田防护林营建模式与经营评价研究

通过对干旱沙区人工绿洲农田防护林林木生长量的调查测定和样本树干解析数据,揭示了林木生长规律,建立了树干材积模型,为农田防护林合理更新提出了科学依据。利用相关依据指标,对现有农田防护林的营建模式进行了评价,提出了新的更新模式。     

基于Biolog-ECO技术不同退耕年限苜蓿地土壤微生物功能多样性分析

运用Biolog-ECO技术,分析半干旱黄土丘陵区不同生长年限紫花苜蓿（Medicago sativa）地和坡耕地土壤微生物功能多样性。结果表明：①在0～15 cm土层,平均吸光值总体变化趋势为生长旺盛苜蓿地显著优于极度退化苜蓿地、退化苜蓿地和坡耕地,坡耕地最差;在15～30 cm土层,平均吸光值总体变化趋势为极度退化...